| Final A | Team | time | -)time | L | React. | 50m | 100m | 150m | | 1. Kimberly Vandenberg | --- | 2:10.54 | | 4 | | 3. | 29.89s | 2. | 1:02.70 | 1. | 1:36.61 | | 2. Lyndsay De Paul | --- | 2:12.34 | 1.80s | 5 | | 1. | 29.53s | 1. | 1:02.60 | 2. | 1:37.33 | | 3. Rita Medrano | --- | 2:12.43 | 1.89s | 3 | | 8. | 31.27s | 6. | 1:04.28 | 3. | 1:37.80 | | 4. Joanna Maranhao | --- | 2:13.00 | 2.46s | 6 | 0.78s | 4. | 30.35s | 3. | 1:03.76 | 4. | 1:37.85 | | 5. Erin Alixe Anne Miller | --- | 2:16.10 | 5.56s | 8 | 0.79s | 2. | 29.74s | 5. | 1:04.20 | 5. | 1:39.36 | | 6. Brenna Gayle Maclean | --- | 2:16.13 | 5.59s | 2 | | 5. | 30.37s | 4. | 1:04.04 | 6. | 1:39.58 | | 7. Eliana Barrios | --- | 2:18.19 | 7.65s | 7 | | 7. | 30.87s | 7. | 1:05.53 | 8. | 1:40.56 | | 8. Prisciliana Escobar | --- | 2:20.16 | 9.62s | 1 | 0.79s | 6. | 30.79s | 8. | 1:06.17 | 7. | 1:40.38 |
| Final B | Team | time | -)time | L | React. | 50m | 100m | 150m | | 9. Yumisleisy Morales | --- | 2:19.78 | | 4 | | 1. | 31.60s | 1. | 1:06.47 | 1. | 1:43.13 | | 10.Amara Gibbs | --- | 2:30.88 | 11.10 | 3 | | 2. | 33.54s | 2. | 1:10.78 | 2. | 1:50.37 | | -. Daiene Dias | --- | DNS | | 5 | | | | | | | |
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| World record: | | Pan American Games record: |
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| Qualification: | | Standard A: 2:19.9 | | Standard B: 2:24.1 |
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| Heats | Team | time | -)time | L | React. | 50m | 100m | 150m | Heat | | 1. Kimberly Vandenberg | --- | 2:12.04 | | 4 | | 3. | 30.48s | 1. | 1:04.01 | 1. | 1:37.95 | Heat 1. | | 2. Lyndsay De Paul | --- | 2:14.17 | 2.13s | 4 | 0.68 | 1. | 29.50s | 2. | 1:04.11 | 3. | 1:39.08 | Heat 2. | | 3. Rita Medrano | --- | 2:14.44 | 2.40s | 5 | | 3. | 31.13s | 3. | 1:05.04 | 2. | 1:39.05 | Heat 2. | | 4. Joanna Maranhao | --- | 2:14.63 | 2.59s | 5 | | 4. | 30.79s | 3. | 1:04.55 | 2. | 1:39.36 | Heat 1. | | 5. Brenna Gayle Maclean | --- | 2:15.87 | 3.83s | 3 | | 2. | 29.97s | 1. | 1:03.65 | 1. | 1:38.80 | Heat 2. | | 6. Eliana Barrios | --- | 2:17.06 | 5.02s | 2 | | 5. | 31.37s | 5. | 1:05.81 | 5. | 1:41.66 | Heat 1. | | 7. Prisciliana Escobar | --- | 2:18.36 | 6.32s | 6 | 0.78 | 2. | 30.32s | 4. | 1:05.29 | 4. | 1:40.32 | Heat 1. | | 8. Erin Alixe Anne Miller | --- | 2:18.37 | 6.33s | 3 | 0.71 | 1. | 30.01s | 2. | 1:04.20 | 3. | 1:39.74 | Heat 1. | | 9. Yumisleisy Morales | --- | 2:19.07 | 7.03s | 2 | | 5. | 32.22s | 5. | 1:06.39 | 4. | 1:41.80 | Heat 2. | | 10.Daiene Dias | --- | 2:19.23 | 7.19s | 6 | 0.73 | 4. | 31.40s | 4. | 1:06.09 | 5. | 1:42.02 | Heat 2. | | 11.Amara Gibbs | --- | 2:30.26 | 18.22s | 7 | | 6. | 33.43s | 6. | 1:10.81 | 6. | 1:50.03 | Heat 1. | | 12.Lara Maria Butler | --- | 2:35.44 | 23.40s | 1 | 0.74 | 6. | 33.36s | 6. | 1:12.33 | 6. | 1:53.25 | Heat 2. | | -. Oriele Espinoza | --- | DNS | | 7 | | | | | | | | Heat 2. |
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