| Estadio Heriberto Jara Corona |
| Final 14:00 | time | | L | React. | Birth | bib | | 1. Lisneidys Inés VeitÍa | CUB | 51.72s | SB | 3 | 0.300 | 29 Apr 1994 | 171 | | 2. Daisiuramis Bonne | CUB | 52.49s | | 6 | 0.218 | 9 Mar 1988 | 143 | | 3. Yenifer Padilla | COL | 52.95s | | 5 | 0.209 | 1 Jan 1990 | 78 | | 4. Nercely Soto | VEN | 53.06s | PB | 4 | 0.387 | 23 Aug 1990 | 486 | | 5. Norma Gonzalez | COL | 53.67s | SB | 2 | 0.228 | 11 Aug 1982 | 67 | | 6. Kineke Alexander | VIN | 54.21s | | 8 | 0.308 | 21 Feb 1986 | 496 | | 7. Rosa Fabian | DOM | 54.75s | 7 | 1 | | 22 Sep 1986 | 196 | | -. Samantha Curtis | JAM | DNS | | 7 | | 8 Sep 1991 | 286 |
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| Temperature: 16°C | | Humidity: 74% | | Conditions: Partly Cloudy |
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| | | First 3 and Best 2 progress to Final. | | Temperature: 17°C ; Humidity: 89% ; Conditions: Rain |
| Heat 1 13:10 | time | | L | React. | Birth | bib | | 1. Daisiuramis Bonne | CUB | 52.88s | | 8 | 0.534 | 9 Mar 1988 | 143 | | 2. Yenifer Padilla | COL | 53.70s | | 7 | 0.229 | 1 Jan 1990 | 78 | | 3. Samantha Curtis | JAM | 55.24s | | 5 | 0.224 | 8 Sep 1991 | 286 | | 4. Mariel Espinoza | MEX | 55.85s | | 4 | 0.328 | 24 Jan 1987 | 347 | | 5. Emileth Pirela | VEN | 55.95s | | 3 | 0.295 | 1 Jan 1993 | 481 | | 6. Ramona Van Der Vloot | SUR | 58.35s | | 6 | 0.231 | 31 Dec 1994 | 438 |
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| Heat 1 13:17 | time | | L | React. | Birth | bib | | 1. Lisneidys Inés VeitÍa | CUB | 52.54s | SB | 6 | 0.309 | 29 Apr 1994 | 171 | | 2. Nercely Soto | VEN | 53.29s | PB | 7 | 0.314 | 23 Aug 1990 | 486 | | 3. Kineke Alexander | VIN | 53.62s | | 3 | 0.221 | 21 Feb 1986 | 496 | | 4. Norma Gonzalez | COL | 53.83s | SB | 5 | 0.234 | 11 Aug 1982 | 67 | | 5. Rosa Fabian | DOM | 54.41s | SB | 5 | | 22 Sep 1986 | 196 | | -. Rossana De Jesus Pardenilla | MEX | DSQ R 163.3 | 8 | 0.249 | 21 Dec 1986 | 368 |
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| Rule 163.3 - Running outside the Lane. |
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