| Final A | 21:31 | time | | | 1. Xandra Velzeboer |  Netherlands | 41.609s | | | 2. Arianna Fontana |  Italy | 42.294s | | | 3. Courtney Sarault |  Canada | 42.427s | | | 4. Selma Poutsma | Netherlands | 42.491s | | | 5. Kim Boutin | Canada | 44.347s | |
| Final B | 21:26 | time | | | 6. Florence Brunelle | Canada | 43.397s | | | 7. Choi Min-jeong |  South Korea | 43.473s | | | 8. Michelle Velzeboer | Netherlands | 1:05.774 | | | 9. Kristen Santos-Griswold |  United States | 1:08.444 | | | 10.Fan Kexin |  China | 1:19.245 | |
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| World Record (broken in Semi-Finals): Xandra Velzeboer, Netherlands 41.416s Salt Lake City, United States 4 November 2022 | | Olympic Record: Suzanne Schulting, Netherlands 42.379s Beijing, China 5 February 2022 |
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| | | First 2 from each Semi advanced to Final. Rest to Final B. |
| Semi 1 | | time | | H | | 1. Xandra Velzeboer | Netherlands | 41.399s | WR | 1 | | 2. Arianna Fontana | Italy | 41.521s | QA | 1 | | 3. Selma Poutsma | Netherlands | 41.760s | qA | 1 | | 4. Kristen Santos-Griswold | United States | 45.364s | QB | 1 | | 5. Michelle Velzeboer | Netherlands | 1:27.632 | QB | 1 |
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| Semi 2 | | time | | H | | 1. Courtney Sarault | Canada | 42.463s | QA | 2 | | 2. Kim Boutin | Canada | 42.672s | QA | 2 | | 3. Fan Kexin | China | 42.833s | QB | 2 | | 4. Florence Brunelle | Canada | 42.838s | QB | 2 | | 5. Choi Min-jeong | South Korea | 43.060s | QB | 2 |
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| Quarter 1 | | time | | H | | 1. Xandra Velzeboer | Netherlands | 41.583s | Q, OR | 1 | | 2. Arianna Fontana | Italy | 41.679s | Q | 1 | | 3. Rika Kanai |  Japan | 43.178s | | 1 | | 4. Bérénice Comby |  France | 56.076s | | 1 | | -. Wang Xinran | China | | PEN | 1 |
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| Quarter 2 | | time | | H | | 1. Courtney Sarault | Canada | 41.974s | Q | 2 | | 2. Florence Brunelle | Canada | 42.285s | Q | 2 | | 3. Fan Kexin | China | 42.737s | q | 2 | | 4. Hanne Desmet |  Belgium | 43.139s | | 2 | | 5. Natalia Maliszewska |  Poland | 58.994s | | 2 |
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| Quarter 3 | | time | | H | | 1. Kristen Santos-Griswold | United States | 42.174s | Q | 3 | | 2. Michelle Velzeboer | Netherlands | 42.417s | Q | 3 | | 3. Kim Gil-li | South Korea | 43.373s | | 3 | | 4. Julie Letai | United States | 1:08.606 | | 3 | | -. Zhang Chutong | China | | PEN | 3 |
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| Quarter 4 | | time | | H | | 1. Choi Min-jeong | South Korea | 41.955s | Q | 4 | | 2. Kim Boutin | Canada | 42.196s | Q | 4 | | 3. Selma Poutsma | Netherlands | 42.234s | q | 4 | | 4. Chiara Betti | Italy | 42.279s | | 4 | | 5. Lee So-yeon | South Korea | 43.330s | | 4 |
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| Heat 1 | | time | | H | | 1. Xandra Velzeboer | Netherlands | 42.417s | Q | 1 | | 2. Chiara Betti | Italy | 43.163s | Q | 1 | | 3. Valentina Aščić |  Croatia | 44.802s | | 1 | | 4. Olga Tikhonova |  Kazakhstan | 46.352s | | 1 |
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| Heat 2 | | time | | H | | 1. Courtney Sarault | Canada | 42.464s | Q | 2 | | 2. Kim Gil-li | South Korea | 43.301s | Q | 2 | | 3. Mirei Nakashima | Japan | 43.655s | | 2 | | 4. Gabriela Topolska | Poland | 43.813s | | 2 |
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| Heat 3 | | time | | H | | 1. Wang Xinran | China | 43.246s | Q | 3 | | 2. Rika Kanai | Japan | 52.160s | Q | 3 | | 3. Arianna Sighel | Italy | 53.960s | | 3 | | 4. Corinne Stoddard | United States | 1:11.651 | | 3 |
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| Heat 4 | | time | | H | | 1. Michelle Velzeboer | Netherlands | 42.588s | Q | 4 | | 2. Arianna Fontana | Italy | 42.668s | Q | 4 | | 3. Bérénice Comby | France | 42.750s | q | 4 | | 4. Yana Khan | Kazakhstan | 1:19.722 | | 4 |
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| Heat 5 | | time | | H | | 1. Kristen Santos-Griswold | United States | 42.767s | Q | 5 | | 2. Zhang Chutong | China | 43.100s | Q | 5 | | 3. Aurélie Lévêque | France | 44.488s | | 5 | | 4. Lea Popovičová |  Slovakia | 46.101s | | 5 |
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| Heat 6 | | time | | H | | 1. Hanne Desmet | Belgium | 43.182s | Q | 6 | | 2. Choi Min-jeong | South Korea | 43.204s | Q | 6 | | 3. Julie Letai | United States | 43.275s | q | 6 | | 4. Yelyzaveta Sydorko |  Ukraine | 43.337s | | 6 |
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| Heat 7 | | time | | H | | 1. Kim Boutin | Canada | 42.692s | Q | 7 | | 2. Natalia Maliszewska | Poland | 43.313s | Q | 7 | | 3. Lee So-yeon | South Korea | 43.406s | q | 7 | | 4. Alena Krylova |  Neutral Athlete | 1:06.997 | | 7 |
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| Heat 8 | | time | | H | | 1. Selma Poutsma | Netherlands | 42.629s | Q | 8 | | 2. Florence Brunelle | Canada | 42.838s | Q | 8 | | 3. Fan Kexin | China | 42.992s | q | 8 | | 4. Diána Laura Végi |  Hungary | 44.164s | | 8 |
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