| Final 10:59 | time |   | gap | L | Reac | 50m |  | 1. Evgeny Rylov |   ROC | 51.98s | ER |   | 2 | 0.55 | 24.96s2. |  | 2. Kliment Kolesnikov |   ROC | 52.00s |   | 0.02s | 5 | 0.62 | 24.90s1. |  | 3. Ryan Murphy |   United States | 52.19s |   | 0.21s | 4 | 0.52 | 25.26s3. |  | 4. Thomas Ceccon |   Italy | 52.30s | NR | 0.32s | 6 | 0.61 | 25.54s6. |  | 5. Jiayu Xu |   China | 52.51s |   | 0.51s | 7 | 0.52 | 25.40s5. |  | 6. Hugo Gonzalez |   Spain | 52.78s |   | 0.80s | 1 | 0.60 | 25.64s=7. |  | 7. Mitch Larkin |   Australia | 52.79s |   | 0.81s | 3 | 0.59 | 25.64s=7. |  | 8. Robert Glinta |   Romania | 52.95s |   | 0.97s | 8 | 0.57 | 25.36s4. |   
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| World and Olympic Record: Ryan Murphy, United States 51.85s Rio de Janeiro, Brazil 13th August 2016 |   
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 Semi-Finals - Monday, 26th July 11:31 |  | Best 8 times advanced to Final.  |   
| Semi 1 11:31 |   | time | gap | L | pts | 50m | age |  | 1. Ryan Murphy |   United States | 52.24s |   | 5 | 977 | 25.39s | 26 |  | 3. Mitchell Larkin |   Australia | 52.76s | +0.52s | 5 | 949 | 25.84s | 28 |  | 4. Thomas Ceccon |   Italy | 52.78s | +0.54s | 3 | 948 | 25.92s | 20 |  | 8. Robert Glinta |   Romania | 53.20s | +0.96s | 6 | 925 | 25.89s | 24 |  | 11.Apostolos Christou |   Greece | 53.41s | +1.17s | 3 | 914 | 25.25s | 24 |  | 13.Marek Ulrich |   Germany | 53.54s | +1.30s | 5 | 908 | 25.80s | 24 |  | 14.Mewen Tomac |   France | 53.62s | +1.38s | 3 | 904 | 25.75s | 19 |  | -. Yohann Ndoye-Brouard |   France | DSQ |   | 2 |   |   | 20 |   
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| Semi 2 |   | time | gap | L | pts | 50m | age |  | 2. Kliment Kolesnikov |   ROC | 52.29s | +0.05s | 4 | 974 | 25.32s | 21 |  | 5. Evgeny Rylov |   ROC | 52.91s | +0.67s | 4 | 941 | 25.75s | 24 |  | 6. Jiayu Xu |   China | 52.94s | +0.70s | 4 | 939 | 25.71s | 25 |  | 7. Hugo Gonzalez |   Spain | 53.05s | +0.81s | 6 | 933 | 25.91s | 22 |  | 9. Ryosuke Irie |   Japan | 53.21s | +0.97s | 2 | 925 | 25.71s | 31 |  | 9. Hunter Armstrong |   United States | 53.21s | +0.97s | 5 | 925 | 25.50s | 20 |  | 12.Isaac Cooper |   Australia | 53.43s | +1.19s | 7 | 913 | 25.61s | 17 |  | 15.Guilherme Guido |   Brazil | 53.80s | +1.56s | 6 | 895 | 25.52s | 34 |   
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| Heat 6 |   | time | gap | L | pts | 50m | age |  | 6. Yohann Ndoye-Brouard |   France | 53.13s | +0.98 | 2 | 929 | 25.97s | 20 |  | 7. Ryan Murphy |   United States | 53.22s | +1.07 | 5 | 924 | 26.15s | 26 |  | 7. Evgeny Rylov |   ROC | 53.22s | +1.07 | 4 | 924 | 26.12s | 24 |  | 12.Robert Glinta |   Romania | 53.67s | +1.52 | 6 | 901 | 26.22s | 24 |  | 15.Apostolos Christou |   Greece | 53.77s | +1.62 | 3 | 896 | 25.78s | 24 |  | 19.Markus Thormeyer |   Canada | 53.80s | +1.65 | 7 | 895 | 26.05s | 23 |  | 26.Cole Pratt |   Canada | 54.27s | +2.12 | 1 | 872 | 26.45s | 18 |  | -. Richard Bohus |   Hungary | DSQ | - | 8 | - | 27.99s | 28 |   
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| Heat 5 |   | time | gap | L | pts | 50m | age |  | 1. Kliment Kolesnikov |   ROC | 52.15s | - | 4 | 982 | 24.89s | 21 |  | 2. Thomas Ceccon |   Italy | 52.49s | +0.34 | 3 | 963 | 25.63s | 20 |  | 4. Mitchell Larkin |   Australia | 52.97s | +0.82 | 5 | 937 | 25.89s | 28 |  | 5. Ryosuke Irie |   Japan | 52.99s | +0.84 | 2 | 936 | 25.64s | 31 |  | 9. Hugo Gonzalez |   Spain | 53.45s | +1.30 | 6 | 912 | 26.13s | 22 |  | 17.Simone Sabbioni |   Italy | 53.79s | +1.64 | 7 | 895 | 26.43s | 24 |  | 20.Juho Lee |   South Korea | 53.84s | +1.69 | 8 | 893 | 25.70s | 26 |  | 24.Pieter Coetze |   South Africa | 54.05s | +1.90 | 1 | 882 | 26.19s | 17 |   
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| Heat 4 |   | time | gap | L | pts | 50m | age |  | 3. Jiayu Xu |   China | 52.70s | +0.55 | 4 | 952 | 25.24s | 25 |  | 10.Mewen Tomac |   France | 53.49s | +1.34 | 3 | 910 | 25.72s | 19 |  | 11.Guilherme Guido |   Brazil | 53.65s | +1.50 | 6 | 902 | 25.62s | 34 |  | 13.Isaac Cooper |   Australia | 53.73s | +1.58 | 7 | 898 | 25.91s | 17 |  | 15.Hunter Armstrong |   United States | 53.77s | +1.62 | 5 | 896 | 26.33s | 20 |  | 17.Luke Greenbank |   Great Britain | 53.79s | +1.64 | 2 | 895 | 26.09s | 23 |  | 29.Adam Telegdy |   Hungary | 54.42s | +2.27 | 8 | 864 | 26.29s | 25 |  | 38.Daniel Martin |   Romania | 56.91s | +4.76 | 1 | 756 | 26.85s | 20 |   
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| Heat 3 |   | time | gap | L | pts | 50m | age |  | 14.Marek Ulrich |   Germany | 53.74s | +1.59 | 5 | 898 | 25.86s | 24 |  | 20.Guilherme Basseto |   Brazil | 53.84s | +1.69 | 8 | 893 | 25.50s | 24 |  | 22.Zheng Wen Quah |   Singapore | 53.94s | +1.79 | 2 | 888 | 25.73s | 24 |  | 25.Ole Braunschweig |   Germany | 54.14s | +1.99 | 4 | 878 | 26.31s | 23 |  | 27.Srihari Nataraj |   India | 54.31s | +2.16 | 6 | 870 | 26.18s | 20 |  | 31.Yakov Toumarkin |   Israel | 54.81s | +2.66 | 7 | 846 | 26.46s | 29 |  | 33.Mikita Tsmyh |   Belarus | 54.88s | +2.73 | 1 | 843 | 26.12s | 24 |  | 35.Bernhard Reitshammer |   Austria | 55.26s | +3.11 | 3 | 826 | 25.95s | 27 |   
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| Heat 2 |   | time | gap | L | pts | 50m | age |  | 23.Kacper Stokowski |   Poland | 53.99s | +1.84 | 6 | 885 | 26.29s | 22 |  | 28.Francisco Rogerio Santos |   Portugal | 54.35s | +2.20 | 2 | 868 | 26.32s | 22 |  | 30.Jan Cejka |   Czechia | 54.69s | +2.54 | 5 | 852 | 26.31s | 20 |  | 32.Dylan Carter |   Trinidad Tobago | 54.82s | +2.67 | 3 | 846 | 26.24s | 25 |  | 36.Michael Laitarovsky |   Israel | 55.34s | +3.19 | 4 | 822 | 25.95s | 21 |  | 37.Kaloyan Levterov |   Bulgaria | 55.60s | +3.45 | 7 | 811 | 26.99s | 18 |   
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| Heat 1 |   | time | gap | L | pts | 50m | age |  | 34.Merdan Atayev |   Turkmenistan | 55.24s | +3.09 | 4 | 826 | 26.48s | 26 |  | 39.Gabriel Castillo |   Bolivia | 58.24s | +6.09 | 5 | 705 | 27.94s | 20 |  | 40.Heriniavo Rasolonjatovo |   Madagascar | 59.81s | +7.66 | 3 | 651 | 28.69s | 23 |   
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